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Single-Strand Annealing Plays a Major Role in Double-Strand DNA Break Repair following CRISPR-Cas9 Cleavage in Leishmania.
Zhang WW, Matlashewski G
mSphere. 2019 Aug 21;4(4). pii: 4/4/e00408-19. doi: 10.1128/mSphere.00408-19.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
123261pLdSaCNExpresses Staphylococcus aureus Cas9 (SaCas9) and its gRNA in Leishmania
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123262pLPhygSaCas9Expresses Staphylococcus aureus Cas9 (SaCas9) in Leishmania
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123265pSPneoSagRNAHExpresses gRNA of Staphylococcus aureus Cas9 (SaCas9) in Leishmania
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123266pNeoSagRNAHExpresses gRNA of Staphylococcus aureus Cas9 (SaCas9) in Leishmania
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