pLdSaCN
(Plasmid #123261)

• Purpose: Expresses Staphylococcus aureus Cas9 (SaCas9) and its gRNA in Leishmania
• Depositing Lab: Greg Matlashewski
• Publication: Zhang et al mSphere. 2019 Aug 21;4(4). pii: 4/4/e00408-19. doi: 10.1128/mSphere.00408-19.

Backbone

• Vector backbone: pSP72
• Total vector size (bp): 8099
• Vector type: CRISPR ; Leishmania
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: gRNA and SaCas9
• Species: Leishmania

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Bbs I (unknown if destroyed)
• 3′ cloning site: Bbs I (unknown if destroyed)
• 5′ sequencing primer: SP6 promoter

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Dr. Feng Zhang Addgene Plasmid pX601
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry