|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||123514||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector typeSynthetic Biology
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert namemPrrn::RBS (3.9%)
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
All inserts have been produced by gene synthesis and cloned in the indicated backbone vector. The insert is a chloroplast promoter::5'UTR fusion designed for Golden-Gate cloning. The Original Material was developed in the performance of research that was funded by Grant Nos. ARPA-E: DE-AR000660 & DARPA: D17AC00016.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pMK-mPrrn::RBS (3.9%) was a gift from Scott Lenaghan & C. Neal Stewart (Addgene plasmid # 123514 ; http://n2t.net/addgene:123514 ; RRID:Addgene_123514)
For your References section:MoChlo: A versatile modular cloning toolbox for chloroplast biotechnology. Occhialini A, Piatek A, Pfotenhauer AC, Frazier TP, Stewart CN, Lenaghan S. Plant Physiol. 2019 Jan 24. pii: pp.18.01220. doi: 10.1104/pp.18.01220. 10.1104/pp.18.01220 PubMed 30679266