MoChlo: A versatile modular cloning toolbox for chloroplast biotechnology.
Occhialini A, Piatek A, Pfotenhauer AC, Frazier TP, Stewart CN, Lenaghan S
Plant Physiol. 2019 Jan 24. pii: pp.18.01220. doi: 10.1104/pp.18.01220. PubMed Article
Occhialini A, Piatek A, Pfotenhauer AC, Frazier TP, Stewart CN, Lenaghan S
Plant Physiol. 2019 Jan 24. pii: pp.18.01220. doi: 10.1104/pp.18.01220. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose | |
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| 123464 | pMK-PaccD (-82 to +23) | MoChlo kit |
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| 123465 | pMK-PaccD (functional) | MoChlo kit |
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| 123466 | pMK-PatpB (NEP functional PEP disabled) | MoChlo kit |
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| 123467 | pMK-PatpB (NEP) | MoChlo kit |
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| 123468 | pMK-PatpB (PEP and NEP functional) | MoChlo kit |
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| 123469 | pMK-PatpB (PEP functional NEP disabled) | MoChlo kit |
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| 123470 | pMK-PatpB (PEP) | MoChlo kit |
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| 123471 | pMK-PatpH | MoChlo kit |
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| 123472 | pMK-PatpI (NEP) | MoChlo kit |
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| 123473 | pMK-PatpI (PEP Hypothetical) | MoChlo kit |
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| 123474 | pMK-PclpP | MoChlo kit |
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| 123475 | pMK-PclpP (PEP) | MoChlo kit |
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| 123476 | pMK-IEE | MoChlo kit |
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| 123477 | pMK-Prrn (-64 to +17) | MoChlo kit |
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| 123478 | pMK-mPrrn (5-fold decrease transcription) | MoChlo kit |
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| 123479 | pMK-PpsbA | MoChlo kit |
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| 123480 | pMK-PpsbA (-42 to +9) | MoChlo kit |
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| 123481 | pMK-PpsbB | MoChlo kit |
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| 123482 | pMK-PpsbD-leader | MoChlo kit |
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| 123483 | pMK-PpsbD-PGTbx | MoChlo kit |
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| 123484 | pMK-PpsbD | MoChlo kit |
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| 123485 | pMK-PrbcL | MoChlo kit |
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| 123486 | pMK-PrbcL (core -35 to +9) | MoChlo kit |
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| 123487 | pMK-PrbcL (-110 to +9) | MoChlo kit |
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| 123488 | pMK-PrpoB | MoChlo kit |
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| 123489 | pMK-Prrn16 | MoChlo kit |
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| 123490 | pMK-Prrn (N. tabacum) | MoChlo kit |
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| 123491 | pMK-Prrn (N. tabacum) APrrn1 | MoChlo kit |
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| 123492 | pMK-Prrn (N. tabacum) APrrn2 | MoChlo kit |
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| 123493 | pMK-Prrn (N. tabacum) APrrn3 | MoChlo kit |
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| 123494 | pMK-Prrn (N. tabacum) APrrn4 | MoChlo kit |
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| 123495 | pMK-Prrn (N. tabacum) APrrn5 | MoChlo kit |
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| 123496 | pMK-Prrn (N. tabacum) APrrn6 | MoChlo kit |
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| 123497 | pMK-Prrn (N. tabacum) APrrn7 | MoChlo kit |
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| 123498 | pMK-Prrn (N. tabacum) APrrn8 | MoChlo kit |
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| 123499 | pMK-Prrn (N. tabacum) APrrn9 | MoChlo kit |
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| 123500 | pMK-Prrn (N. tabacum) APrrn10 | MoChlo kit |
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| 123501 | pMK-Prrn (N. tabacum) BPrrn12 | MoChlo kit |
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| 123502 | pMK-Prrn (N. tabacum) BPrrn13 | MoChlo kit |
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| 123503 | pMK-Prrn (N. tabacum) BPrrn14 | MoChlo kit |
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| 123504 | pMK-Prrn (N. tabacum) BPrrn15 | MoChlo kit |
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| 123505 | pMK-Prrn (N. tabacum) BPrrn16 | MoChlo kit |
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| 123506 | pMK-Prrn (N. tabacum) BPrrn17 | MoChlo kit |
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| 123507 | pMK-Prrn (N. tabacum) BPrrn18 | MoChlo kit |
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| 123508 | pMK-Prrn (N. tabacum) BPrrn19 | MoChlo kit |
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| 123509 | pMK-Prrn (N. tabacum) BPrrn20 | MoChlo kit |
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| 123510 | pMK-Prrn (N. tabacum) BPrrn21 | MoChlo kit |
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| 123511 | pMK-Zm PclpP::5'UTR | MoChlo kit |
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| 123512 | pMK-Zm PclpP::Gg10 leader | MoChlo kit |
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| 123513 | pMK-PaccD::5'UTR | MoChlo kit |
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| 123514 | pMK-mPrrn::RBS (3.9%) | MoChlo kit |
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| 123515 | pOK-mPrrn::RBS (20%) | MoChlo kit |
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| 123516 | pMK-mPrrn::RBS (46%) | MoChlo kit |
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| 123517 | pMK-mPrrn::RBS (60%) | MoChlo kit |
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| 123518 | pMK-mPrrn::RBS (61%) | MoChlo kit |
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| 123519 | pMK-Prrn::RBS | MoChlo kit |
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| 123520 | pMK-atpB 5'UTR | MoChlo kit |
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| 123521 | pMK-atpB 5'UTR +10 (functional) | MoChlo kit |
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| 123522 | pMK-atpB 5'UTR +10 processed -90 (functional) | MoChlo kit |
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| 123523 | pMK-atpB 5'UTR TCR processed -90 (functional) | MoChlo kit |
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| 123524 | pMK-atpH 5'UTR | MoChlo kit |
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| 123525 | pMK-atpH 5'UTR processed -45 | MoChlo kit |
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| 123526 | pMK-atpI 5'UTR | MoChlo kit |
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| 123527 | pMK-psbN 5'UTR | MoChlo kit |
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| 123528 | pMK-psbN 5'UTR processed -39 | MoChlo kit |
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| 123529 | pMK-psbB 5'UTR | MoChlo kit |
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| 123530 | pMK-psbB 5'UTR processed -54 | MoChlo kit |
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| 123531 | pMK-ndhD 5'UTR | MoChlo kit |
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| 123532 | pMK-rps2 5'UTR | MoChlo kit |
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| 123533 | pMK-rps2 5'UTR mAAGA | MoChlo kit |
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| 123534 | pMK-rps2 5'UTR mCCUC | MoChlo kit |
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| 123535 | pMK-rps2 5'UTR mAAGA (-20 to -10 deletion) | MoChlo kit |
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| 123536 | pMK-rps2 5'UTR mCCUC (-20 to -10 deletion) | MoChlo kit |
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| 123537 | pMK-psbH 5'UTR + start codon + 1AA | MoChlo kit |
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| 123538 | pMK-psbA 5'UTR (SNM) | MoChlo kit |
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| 123539 | pMK-psbA 5'UTR (SEM) | MoChlo kit |
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| 123540 | pMK-psbA 5'UTR (delta SL) | MoChlo kit |
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| 123541 | pMK-psbA 5'UTR (delta 5'SL) | MoChlo kit |
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| 123542 | pMK-psbA-rbcL chimeric 5'UTR (R delta 5'SL) | MoChlo kit |
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| 123543 | pMK-psbA-rbcL chimeric 5'UTR (R'SLWT) | MoChlo kit |
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| 123544 | pMK-rps14 5'UTR | MoChlo kit |
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| 123545 | pMK-rbcL 5'UTR (full) | MoChlo kit |
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| 123546 | pMK-rbcL 5'UTR (processed) | MoChlo kit |
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| 123547 | pMK-rbcL 5'UTR + 14 AA | MoChlo kit |
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| 123548 | pMK-T7G10 leader | MoChlo kit |
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| 123549 | pMK-rrn16 5'UTR (native) | MoChlo kit |
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| 123550 | pMK-rrn16 5'UTR (+37) | MoChlo kit |
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| 123551 | pMK-psbA 5'UTR | MoChlo kit |
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| 123552 | pMK-Synthetic RBS | MoChlo kit |
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| 123553 | pMK-psbC 5'UTR | MoChlo kit |
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| 123554 | pMK-accD 5'UTR | MoChlo kit |
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| 123555 | pMK-clpP 5'UTR | MoChlo kit |
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| 123556 | pMK-mEmerald | MoChlo kit |
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| 123557 | pMK-pporRFP | MoChlo kit |
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| 123558 | pMK-aadA | MoChlo kit |
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| 123559 | pMK-aphA6 | MoChlo kit |
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| 123560 | pMK-nptII | MoChlo kit |
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| 123561 | pMK-Myc aadA-GFP | MoChlo kit |
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| 123562 | pMK-FLARE16-S aadA-GFP | MoChlo kit |
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| 123563 | pMK-Lux-C codon opt. Nt chloroplasts | MoChlo kit |
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| 123564 | pMK-Lux-D codon opt. Nt chloroplasts | MoChlo kit |
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| 123565 | pMK-Lux-A codon opt. Nt chloroplasts | MoChlo kit |
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| 123566 | pMK-Lux-B codon opt. Nt chloroplasts | MoChlo kit |
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| 123567 | pMK-Lux-E codon opt. Nt chloroplasts | MoChlo kit |
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| 123568 | pMK-Lux-G codon opt. Nt chloroplasts | MoChlo kit |
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| 123569 | pMK-psbA 3'UTR | MoChlo kit |
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| 123570 | pMK-rbcL 3'UTR | MoChlo kit |
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| 123571 | pMK-petD 3'UTR | MoChlo kit |
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| 123572 | pMK-rpoA 3'UTR | MoChlo kit |
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| 123573 | pMK-E.coli rrnB 3'UTR | MoChlo kit |
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| 123574 | pMK-psaC 3'UTR | MoChlo kit |
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| 123575 | pMK-TMV 3'UTR | MoChlo kit |
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| 123576 | pMK-TYMV 3'UTR | MoChlo kit |
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| 123577 | pMK-BMV 3'UTR | MoChlo kit |
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| 123578 | pMK-ALMV 3'UTR | MoChlo kit |
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| 123579 | pMK-N. tabacum trnI/trnA | MoChlo kit |
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| 123580 | pMK-N. tabacum trnG/trnfM | MoChlo kit |
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| 123581 | pMK-N. tabacum trnV/3'rps12 | MoChlo kit |
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| 123582 | pMK-N. tabacum atpB/accD | MoChlo kit |
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| 123583 | pMK-S. tuberosum trnI/trnA | MoChlo kit |
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| 123584 | pMK-S. tuberosum trnG/trnfM | MoChlo kit |
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| 123585 | pMK-S. tuberosum trnV/3'rps12 | MoChlo kit |
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