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pEW4: pET-32a Dot1L(2-386)
(Plasmid #124099)


Item Catalog # Description Quantity Price (USD)
Plasmid 124099 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
  • Backbone size w/o insert (bp) 5888
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number


  • Gene/Insert name
  • Species
    H. sapiens (human)
  • Insert Size (bp)
  • Entrez Gene
    DOT1L (a.k.a. DOT1, KMT4)
  • Promoter T7
  • Tag / Fusion Protein
    • Trx-His-TEV (N terminal on backbone)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEW4: pET-32a Dot1L(2-386) was a gift from Cynthia Wolberger (Addgene plasmid # 124099 ; ; RRID:Addgene_124099)
  • For your References section:

    Mechanism of Cross-talk between H2B Ubiquitination and H3 Methylation by Dot1L. Worden EJ, Hoffmann NA, Hicks CW, Wolberger C. Cell. 2019 Feb 8. pii: S0092-8674(19)30151-5. doi: 10.1016/j.cell.2019.02.002. 10.1016/j.cell.2019.02.002 PubMed 30765112