6107 pETH6KT218
(Plasmid
#1241)
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 1241 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepET28a
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Backbone manufacturerNovagen
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameHsp104 (K218T)
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Alt nameHsp104
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SpeciesS. cerevisiae (budding yeast)
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MutationK218T
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Entrez GeneHSP104 (a.k.a. YLL026W)
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Tag
/ Fusion Protein
- His tag (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site nde1 (not destroyed)
- 3′ cloning site sac1 (not destroyed)
- 5′ sequencing primer n/a (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
6107 pETH6KT218 was a gift from Susan Lindquist (Addgene plasmid # 1241 ; http://n2t.net/addgene:1241 ; RRID:Addgene_1241) -
For your References section:
Hsp104 catalyzes formation and elimination of self-replicating Sup35 prion conformers. Shorter J, Lindquist S. Science 2004 Jun 18;304(5678):1793-7. Epub 2004 May 20. 10.1126/science.1098007 PubMed 15155912
