pPICZ-mouseGIRK2R201A-eGFP-StII-6xHis
(Plasmid #124278)

• Purpose: Expresses mouse GIRK2 R201A as a TEV protease cleavable C-terminal fusion to eGFP, strep tag II, and 6x His tag
• Depositing Lab: Arthur Laganowsky
• Publication: Liu et al Nat Commun. 2019 Mar 22;10(1):1352. doi: 10.1038/s41467-019-09333-4.

Backbone

• Vector backbone: pPICZ
• Backbone manufacturer: Invitrogen
• Vector type: Yeast Expression
• Selectable markers: Zeocin

Growth in Bacteria

• Bacterial Resistance(s): Bleocin (Zeocin), 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: GIRK2
• Species: M. musculus (mouse)
• Mutation: R201A
• Entrez Gene: Kcnj6 (a.k.a. BIR1, GIRK2, KATP2, KCNJ7, Kir3.2, weaver, wv)
• Promoter: AOX1
• Tag / Fusion Protein:
  • eGFP-StreptagII-6xHis (C terminal on insert)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: GAC TGG TTC CAA TTG ACA AGC
• 3′ sequencing primer: GCA AAT GGC ATT CTG ACA TCC

Resource Information

• Supplemental Documents:
  • pPICZB_mGIRK2R201A_TEV_eGFP_StII_6xHis.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry