pSuperRetro-EFP siRNA2
(Plasmid #12450)

• Depositing Lab: Dong-Er Zhang
• Publication: Zou et al J Biol Chem. 2006 Feb 17. 281(7):3989-94.

Backbone

• Vector backbone: pSuperRetroPuro
• Backbone size w/o insert (bp): 6349
• Vector type: Mammalian Expression, Retroviral, RNAi
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: siRNA against EFP
• Alt name: EFP
• Species: H. sapiens (human)
• Entrez Gene: TRIM25 (a.k.a. EFP, RNF147, Z147, ZNF147)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (not destroyed)
• 3′ cloning site: BglII (not destroyed)
• 5′ sequencing primer: pBluescript KS

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The targeted sequence for siRNA is EFP cDNA region 711-729 (ggtggagcagctacaacaa). The synthesized oligonucleotides is 5'-Gatccccggtggagcagctacaacaattcaag agattgttgtagctgctccaccttttta-3' and 5'-Agcttaaaaaggtggagcagctacaacaatct cttgaattgttgtagctgctccaccggg-3'.