pHO_pTEFmut7_CggR_R250A_ble
(Plasmid #124585)

• Purpose: Plasmid for genomic integration of CggR_R250A repressor regulated by the pTEFmut7 promoter
• Depositing Lab: Matthias Heinemann
• Publication: Monteiro et al Mol Syst Biol. 2019 Dec;15(12):e9071. doi: 10.15252/msb.20199071.

Backbone

• Vector backbone: pHO - plasmid
• Modifications to backbone: Insertion of the ble selection marker (resistance against phleomycin)
• Vector type: Synthetic Biology
• Selectable markers: Phleomycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: cggR_R250A
• Species: Synthetic
• Mutation: changed arginine 250 to alanine
• Entrez Gene: cggR (a.k.a. BSU_33950, BSU33950)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: AAA ACT ATG GCT CAA GCA AGA AAC ACC CCA
• 3′ sequencing primer: TTT TGA TAC CGA GTT CGT TCT TTG TGG GGT

Resource Information

• Supplemental Documents:
  • pHO_pTEFmut7_CggR_R250A_ble.gb
• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please visit https://www.biorxiv.org/content/10.1101/682302v1 for BioRxiv preprint