pTEF6-7
(Plasmid #124583)

• Purpose: Reporter plasmid with constitutively expressed mCherry and YFP (eCitrine)
• Depositing Lab: Matthias Heinemann
• Publication: Monteiro et al Mol Syst Biol. 2019 Dec;15(12):e9071. doi: 10.15252/msb.20199071.

Backbone

• Vector backbone: p416
• Vector type: Yeast Expression
• Selectable markers: Neomycin (select with G418), URA3

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: mCherry

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: KpnI (not destroyed)
• 3′ cloning site: KpnI (not destroyed)
• 5′ sequencing primer: ATTAGGTACCCGAGCTCTCGAGAACCCTTAATATAACTT
• 3′ sequencing primer: ATATGGTACCAGCGTACGAGCGACCTCATG

Resource Information

• Supplemental Documents:
  • TEF6-7_two_color_plasmid.gb
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please visit https://www.biorxiv.org/content/10.1101/682302v1 for BioRxiv preprint