Human SHIP2
(Plasmid #124645)

• Purpose: Expresses HIS tagged human SHIP2 in COS-7/HEK cells
• Depositing Lab: Christophe Erneux
• Publication: Pesesse et al J Biol Chem. 2001 Jul 27;276(30):28348-55. doi: 10.1074/jbc.M103537200. Epub 2001 May 10.

Backbone

• Vector backbone: pcDNA3 HIS C
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5514
• Total vector size (bp): 9978
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: INPPL1
• Alt name: SHIP2; 51C protein
• Species: H. sapiens (human)
• Insert Size (bp): 4503
• Mutation: coding sequence starts at amino acid 18 as calculated from the 1258 amino acids full length sequence in Pesesse et al Biochem Biophys Res Commun. 1997 Oct 29;239(3):697-700 "Identification of a second SH2-domain-containing protein closely related to the phosphatidylinositol polyphosphate 5-phosphatase SHIP".
• GenBank ID: NM_001567
• Entrez Gene: INPPL1 (a.k.a. OPSMD, SHIP2)
• Tag / Fusion Protein:
  • His Xpress (N-terminal) (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: T3

Resource Information

• Supplemental Documents:
  • Starting sequence of this plamid at A18 of SHIP2 1258 amino acid protein
  • MAP of SHIP2 in pcDNA3HIS

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry