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Human SHIP1 (clone hp51CN)
(Plasmid #123347)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 123347 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 123347-D50 50 μg of DNA in Tris buffer $495
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DNA 123347-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pcDNA3
  • Backbone size w/o insert (bp) 5446
  • Total vector size (bp) 9450
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    INPP5D
  • Alt name
    SHIP
  • Alt name
    SHIP1
  • Alt name
    hp51CN; p150Ship; SHIP; SHIP-1; SHIP1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    4000
  • GenBank ID
    NM_005541 EMBL sequence data base X98429
  • Entrez Gene
    INPP5D (a.k.a. SHIP, SHIP-1, SHIP1, SIP-145, hp51CN, p150Ship)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRV (unknown if destroyed)
  • 3′ cloning site XbaI (unknown if destroyed)
  • 5′ sequencing primer M13 forward primer
  • 3′ sequencing primer M13 reverse primer
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Digestion of this plasmid with EcoRI and XhoI should yield three bands of 1.75, 2.1 and 5.6 kb

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Human SHIP1 (clone hp51CN) was a gift from Christophe Erneux (Addgene plasmid # 123347 ; http://n2t.net/addgene:123347 ; RRID:Addgene_123347)

  • For your References section:

    Cloning and expression of a human placenta inositol 1,3,4,5-tetrakisphosphate and phosphatidylinositol 3,4,5-trisphosphate 5-phosphatase. Drayer AL, Pesesse X, De Smedt F, Woscholski R, Parker P, Erneux C. Biochem Biophys Res Commun. 1996 Aug 5;225(1):243-9. doi: 10.1006/bbrc.1996.1161. 10.1006/bbrc.1996.1161 PubMed 8769125
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