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nLuc-GLP-1R-His-FLAG
(Plasmid #124831)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 124831 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 124831-D50 50 μg of DNA in Tris buffer $495
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DNA 124831-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pSectag2
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5200
  • Total vector size (bp) 7200
  • Vector type
    Mammalian Expression, Luciferase
  • Selectable markers
    Zeocin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    GLP1R
  • Alt name
    Glucagon-like peptide-1 receptor
  • Species
    H. sapiens (human), Synthetic
  • Insert Size (bp)
    1959
  • GenBank ID
    AAI12127.1
  • Entrez Gene
    GLP1R (a.k.a. GLP-1, GLP-1-R, GLP-1R)
  • Promoter CMV
  • Tags / Fusion Proteins
    • NanoLuc (N terminal on insert)
    • His6 (C terminal on insert)
    • FLAG (C terminal on insert)

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    DNA encoding Nanoluc was kindly provided by Promega. GLP-1R cloned in a modifed pSectag2 was kindly provided by Pfizer (Nolte, et al. “A Potentiator of Orthosteric Ligand Activity at GLP-1R Acts via Covalent Modification.” Nat. Chem. Biol. 10, no. 8 (2014): 629–31.) .

Terms and Licenses

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    nLuc-GLP-1R-His-FLAG was a gift from Sam Gellman (Addgene plasmid # 124831 ; http://n2t.net/addgene:124831 ; RRID:Addgene_124831)

  • For your References section:

    Structural and functional diversity among agonist-bound states of the GLP-1 receptor. Cary BP, Deganutti G, Zhao P, Truong TT, Piper SJ, Liu X, Belousoff MJ, Danev R, Sexton PM, Wootten D, Gellman SH. Nat Chem Biol. 2022 Mar;18(3):256-263. doi: 10.1038/s41589-021-00945-w. Epub 2021 Dec 22. 10.1038/s41589-021-00945-w PubMed 34937906
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