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(Plasmid #124831)


Item Catalog # Description Quantity Price (USD)
Plasmid 124831 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
  • Backbone size w/o insert (bp) 5200
  • Total vector size (bp) 7200
  • Vector type
    Mammalian Expression, Luciferase
  • Selectable markers

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number


  • Gene/Insert name
  • Alt name
    Glucagon-like peptide-1 receptor
  • Species
    H. sapiens (human), Synthetic
  • Insert Size (bp)
  • GenBank ID
  • Entrez Gene
    GLP1R (a.k.a. GLP-1, GLP-1-R, GLP-1R)
  • Promoter CMV
  • Tags / Fusion Proteins
    • NanoLuc (N terminal on insert)
    • His6 (C terminal on insert)
    • FLAG (C terminal on insert)

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    DNA encoding Nanoluc was kindly provided by Promega. GLP-1R cloned in a modifed pSectag2 was kindly provided by Pfizer (Nolte, et al. “A Potentiator of Orthosteric Ligand Activity at GLP-1R Acts via Covalent Modification.” Nat. Chem. Biol. 10, no. 8 (2014): 629–31.) .

Terms and Licenses

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    nLuc-GLP-1R-His-FLAG was a gift from Sam Gellman (Addgene plasmid # 124831 ; ; RRID:Addgene_124831)
  • For your References section:

    Structural and functional diversity among agonist-bound states of the GLP-1 receptor. Cary BP, Deganutti G, Zhao P, Truong TT, Piper SJ, Liu X, Belousoff MJ, Danev R, Sexton PM, Wootten D, Gellman SH. Nat Chem Biol. 2022 Mar;18(3):256-263. doi: 10.1038/s41589-021-00945-w. Epub 2021 Dec 22. 10.1038/s41589-021-00945-w PubMed 34937906