pFB1.HMBP.PrS.AEBP2
(Plasmid #125165)

• Purpose: Expresses human AEBP2 in insect cells, , under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag.
• Depositing Lab: Chen Davidovich
• Publication: Zhang et al Nat Struct Mol Biol. 2019 Mar;26(3):237-247. doi: 10.1038/s41594-019-0197-y. Epub 2019 Mar 4.

Backbone

• Vector backbone: pFB1.HMBP.A3.PrS.ybbR (parent pFast.Bac1)
• Backbone size w/o insert (bp): 5986
• Modifications to backbone: N-terminal His-MBP PreScission cleavage site
• Vector type: Insect Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Zinc finger protein AEBP2
• Alt name: Adipocyte enhancer-binding protein 2
• Species: H. sapiens (human)
• Insert Size (bp): 1512
• GenBank ID: 121536
• Entrez Gene: AEBP2
• Promoter: Polyhedrin
• Tag / Fusion Protein:
  • His-MBP (N terminal on backbone)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: U003F pFB1 GGATTATTCATACCGTCCCA
• 3′ sequencing primer: U004 [Univ. pFB1/pFBD[PH] R] CAAATGTGGTATGGCTGATT

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry