pFB1.HMBP.PrS.EZH2
(Plasmid #125161)

• Purpose: Expresses human EZH2 in insect cells, , under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag.
• Depositing Lab: Chen Davidovich
• Publication: Zhang et al Nat Struct Mol Biol. 2019 Mar;26(3):237-247. doi: 10.1038/s41594-019-0197-y. Epub 2019 Mar 4.

Backbone

• Vector backbone: pFast.Bac1 (pFB1.HMBP.A3.PrS.ybbR)
• Backbone size w/o insert (bp): 5986
• Modifications to backbone: N-terminal His-MBP PreScission cleavage site
• Vector type: Insect Expression ; baculovirus expression vector

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: EZH2
• Alt name: Histone-lysine N-methyltransferase EZH2
• Species: H. sapiens (human)
• Insert Size (bp): 2256
• GenBank ID: XM_017011817.2 2146
• Entrez Gene: EZH2 (a.k.a. ENX-1, ENX1, EZH2b, KMT6, KMT6A, WVS, WVS2)
• Tag / Fusion Protein:
  • His-MBP (N terminal on backbone)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry