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pBabe puro HA PIK3CB
(Plasmid #12526)


Item Catalog # Description Quantity Price (USD)
Plasmid 12526 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
    pBabe puro
  • Backbone manufacturer
    Available at Addgene (plasmid #1764)
  • Backbone size w/o insert (bp) 5150
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
  • Alt name
    phosphoinositide 3-kinase
  • Alt name
  • Alt name
    p110 beta
  • Species
    H. sapiens (human)
  • Insert Size (bp)
  • Entrez Gene
    PIK3CB (a.k.a. P110BETA, PI3K, PI3KBETA, PIK3C1)
  • Tag / Fusion Protein
    • HA (N terminal on insert)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

HMS clone ID number: HsCD00025922. To construct a hemagglutinin (HA)-tagged allele of p110 beta, a cDNA clone of human PIK3CB (obtained from the FLEXGene Repository of the Harvard Institute of Proteomics) was subcloned into pBabepuro by PCR with primers containing HA-epitope coding sequence with subsequent restriction and ligation.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBabe puro HA PIK3CB was a gift from Jean Zhao (Addgene plasmid # 12526 ; ; RRID:Addgene_12526)
  • For your References section:

    The oncogenic properties of mutant p110alpha and p110beta phosphatidylinositol 3-kinases in human mammary epithelial cells. Zhao JJ, Liu Z, Wang L, Shin E, Loda MF, Roberts TM. Proc Natl Acad Sci U S A. 2005 Dec 20. 102(51):18443-8. 10.1073/pnas.0508988102 PubMed 16339315