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(Plasmid #125263)


Item Catalog # Description Quantity Price (USD)
Plasmid 125263 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone size w/o insert (bp) 9075
  • Total vector size (bp) 9852
  • Vector type
    Mammalian Expression, Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
    NEB Stable
  • Copy number


  • Gene/Insert name
  • Species
    H. sapiens (human)
  • Insert Size (bp)
  • Promoter EF1a
  • Tag / Fusion Protein
    • NLS (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer tgaagttaggccagcttgg
  • 3′ sequencing primer cattaaagcagcgtatcc
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    CSII-prEF1a-E2-Crimson-NLS was a gift from Jan Skotheim (Addgene plasmid # 125263 ; ; RRID:Addgene_125263)
  • For your References section:

    Constitutive expression of a fluorescent protein reports the size of live human cells. Berenson DF, Zatulovskiy E, Xie S, Skotheim JM. Mol Biol Cell. 2019 Nov 15;30(24):2985-2995. doi: 10.1091/mbc.E19-03-0171. Epub 2019 Oct 10. 10.1091/mbc.E19-03-0171 PubMed 31599704