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(Plasmid #112244)


Item Catalog # Description Quantity Price (USD)
Plasmid 112244 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone size w/o insert (bp) 5500
  • Total vector size (bp) 12204
  • Modifications to backbone
    Hygromycin resistance
  • Vector type
    Mammalian Expression
  • Selectable markers

Growth in Bacteria

  • Bacterial Resistance(s)
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
  • Species
    S. pyogenes
  • Insert Size (bp)
  • Mutation
    NS3 mutant T54A
  • Promoter CMV
  • Tags / Fusion Proteins
    • HA (C term on NS3)
    • AU1 (N term on NS3)

Cloning Information

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    dCas9-VPR was adapted from Chavez et al., 2015 (Addgene plasmid #63798) and NS3 was adapted from the Roger Tsien lab.
  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    dCas9-NS3-NLS/VPR was a gift from John Ngo (Addgene plasmid # 112244 ; ; RRID:Addgene_112244)
  • For your References section:

    Chemogenetic control of gene expression and cell signaling with antiviral drugs. Tague EP, Dotson HL, Tunney SN, Sloas DC, Ngo JT. Nat Methods. 2018 Jul;15(7):519-522. doi: 10.1038/s41592-018-0042-y. Epub 2018 Jul 2. 10.1038/s41592-018-0042-y PubMed 29967495