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Chemogenetic control of gene expression and cell signaling with antiviral drugs.
Tague EP, Dotson HL, Tunney SN, Sloas DC, Ngo JT
Nat Methods. 2018 Jul;15(7):519-522. doi: 10.1038/s41592-018-0042-y. Epub 2018 Jul 2.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
112244dCas9-NS3-NLS/VPREncodes the drug-preservable Cas9-NS3-NLS/VPR, which can be used to activate gene expression when combined with an NS3 inhibitor and appropriately designed sgRNA.
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112279ss-SNAP-TMD-NS3-Gal4Encodes the membrane-targeted TMD-NS3-Gal4, which exhibits drug-inducible transcription "turn-off" activity. SNAP-tag is fused to the extracellular region of the encoded protein construct.
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112627Gal4DB-NS3-VP64-p65_IRES_mCherryEncodes the drug-activated transcription factor Gal4DB-NS3-VP64-p65 along with IRES mCherry as a fluorescent marker.
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112628rTetR-NS3-VP64-p65_IRES_mCherryEncodes the drug-activated transcription factor rTetR-NS3-VP64-p65 along with IRES mCherry as a fluorescent marker.
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