|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||12567||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 7200
Vector typeMammalian Expression, Luciferase ; Reporter for translation repression
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameOne perfect CXCR4 binding site
SpeciesH. sapiens (human)
Insert Size (bp)40
Entrez GeneCXCR4 (a.k.a. CD184, D2S201E, FB22, HM89, HSY3RR, LAP-3, LAP3, LCR1, LESTR, NPY3R, NPYR, NPYRL, NPYY3R, WHIM, WHIMS)
/ Fusion Protein
- luciferase (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site XbaI (not destroyed)
- 3′ cloning site ApaI (not destroyed)
- 5′ sequencing primer n/a
- 3′ sequencing primer BGH rev primer (Common Sequencing Primers)
Firefly luciferase was cloned into the HindIII/SpeI sites of pcDNA3.1. One perfect complementary binding site to the antisense strand of CXCR4 siRNA was cloned in the XbaI/ApaI sites in the 3'UTR of luciferase.
Perfect binding site sequence is - 5'-AAGTTTTCACTCCAGCTAACA
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:PCD FL1P was a gift from Carl Novina (Addgene plasmid # 12567 ; http://n2t.net/addgene:12567 ; RRID:Addgene_12567)
For your References section:Recapitulation of short RNA-directed translational gene silencing in vitro. Wang B, Love TM, Call ME, Doench JG, Novina CD. Mol Cell. 2006 May 19. 22(4):553-60. 10.1016/j.molcel.2006.03.034 PubMed 16713585
Map uploaded by the depositor.