Cytosolic Syk Biosensor
(Plasmid #125729)

• Purpose: FRET biosensor for studying SYK activities in cells
• Depositing Lab: Yingxiao Wang
• Publication: Xiang et al Cell Mol Bioeng. 2011 Dec;4(4):670-677. doi: 10.1007/s12195-011-0211-x.

Backbone

• Vector backbone: pcDNA3.1’
• Backbone manufacturer: Invitrogen, modified at Dr. Roger Tsien Lab in University of California, San Diego
• Backbone size w/o insert (bp): 5505
• Total vector size (bp): 6287
• Modifications to backbone: The original fragment between HindIII and EcoRI in pcDNA3.1 is replaced by the following fragment “GCGGCCGCCACCATG GTG CGG GGT TCT CATCAT CAT CATCATCATGGTATGGCT AGC ATG ACT GGT GGA CAG CAA ATG GGT CCG GAT CTG TAC GAC GAT AAG GAT CCC AGC TCG AGA TCT GCA GCT GGT ACC ATG “ containing a Kozak sequence and 6xHistine tag.
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: SYK biosensor
• Alt name: ZAP70 biosensor
• Species: Synthetic
• Insert Size (bp): 1782
• Entrez Gene: ZAP70 (a.k.a. ADMIO2, IMD48, SRK, STCD, STD, TZK, ZAP-70)
• Tag / Fusion Protein:
  • 6xHistine Tag at the N-terminal.

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry