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Cytosolic Syk Biosensor
(Plasmid #125729)


Item Catalog # Description Quantity Price (USD)
Plasmid 125729 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
    Invitrogen, modified at Dr. Roger Tsien Lab in University of California, San Diego
  • Backbone size w/o insert (bp) 5505
  • Total vector size (bp) 6287
  • Modifications to backbone
    The original fragment between HindIII and EcoRI in pcDNA3.1 is replaced by the following fragment “GCGGCCGCCACCATG GTG CGG GGT TCT CATCAT CAT CATCATCATGGTATGGCT AGC ATG ACT GGT GGA CAG CAA ATG GGT CCG GAT CTG TAC GAC GAT AAG GAT CCC AGC TCG AGA TCT GCA GCT GGT ACC ATG “ containing a Kozak sequence and 6xHistine tag.
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
    SYK biosensor
  • Alt name
    ZAP70 biosensor
  • Species
  • Insert Size (bp)
  • Entrez Gene
    ZAP70 (a.k.a. ADMIO2, IMD48, SRK, STCD, STD, TZK, ZAP-70)
  • Tag / Fusion Protein
    • 6xHistine Tag at the N-terminal.

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Cytosolic Syk Biosensor was a gift from Yingxiao Wang (Addgene plasmid # 125729 ; ; RRID:Addgene_125729)
  • For your References section:

    A FRET-Based Biosensor for Imaging SYK Activities in Living Cells. Xiang X, Sun J, Wu J, He HT, Wang Y, Zhu C. Cell Mol Bioeng. 2011 Dec;4(4):670-677. doi: 10.1007/s12195-011-0211-x. 10.1007/s12195-011-0211-x PubMed 25541586