pBabe puro H-Ras 12V (40C)
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||12590||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector backbonepBabe puro
- Backbone size w/o insert (bp) 5169
Vector typeMammalian Expression, Retroviral
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameHRas 12V 40C
SpeciesH. sapiens (human)
Insert Size (bp)500
Entrez GeneHRAS (a.k.a. C-BAS/HAS, C-H-RAS, C-HA-RAS1, CTLO, H-RASIDX, HAMSV, HRAS1, RASH1, p21ras)
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer pBABE 5'
- 3′ sequencing primer pBABE 3' (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pBabe puro H-Ras 12V (40C) was a gift from Channing Der (Addgene plasmid # 12590 ; http://n2t.net/addgene:12590 ; RRID:Addgene_12590)
For your References section:Oncogenic Ras blocks anoikis by activation of a novel effector pathway independent of phosphatidylinositol 3-kinase. McFall A, Ulku A, Lambert QT, Kusa A, Rogers-Graham K, Der CJ. Mol Cell Biol. 2001 Aug . 21(16):5488-99. 10.1128/MCB.21.16.5488-5499.2001 PubMed 11463831
Map uploaded by the depositor.