pRK DPC4 Flag (aka: Smad4 Flag)
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||12627||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4700
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
SpeciesH. sapiens (human)
Entrez GeneSMAD4 (a.k.a. DPC4, JIP, MADH4, MYHRS)
/ Fusion Protein
- flag (C terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site SalI (not destroyed)
- 5′ sequencing primer SP6 (Common Sequencing Primers)
PCR'd human placental library to get 5' end of DPC4 sequences using primers, then combined with an insert of Image clone 23275 to get full-length DPC4. See author's map for details.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pRK DPC4 Flag (aka: Smad4 Flag) was a gift from Rik Derynck (Addgene plasmid # 12627 ; http://n2t.net/addgene:12627 ; RRID:Addgene_12627)
For your References section:Receptor-associated Mad homologues synergize as effectors of the TGF-beta response. Zhang Y, Feng X, We R, Derynck R. Nature. 1996 Sep 12. 383(6596):168-72. 10.1038/383168a0 PubMed 8774881
Map uploaded by the depositor.