pJHG004
(Plasmid #126455)

• Purpose: Expresses dTdT in mammalian cells.
• Depositing Lab: Chang Liu
• Depositing Organization: University of California, Irvine
  Located in United States of America
• Publication: Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. doi: 10.1038/s41589-021-00769-8. Epub 2021 Mar 22.

Backbone

• Vector backbone: pcDNA3.1
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5523
• Total vector size (bp): 7053
• Modifications to backbone: Added a Kozak consensus sequence downstream of the NheI site. Switched neomycin resistance to hygromycin.
• Vector type: Mammalian Expression
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: dTdT
• Alt name: terminal deoxynucleotidyl transferase
• Alt name: DNA nucleotidylexotransferase
• Species: H. sapiens (human)
• Insert Size (bp): 1530
• Mutation: Added D343E and D345E to inactivate the enzyme.
• GenBank ID: NM_004088.3
• Entrez Gene: DNTT (a.k.a. TDT)
• Promoter: CMV

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: CMV Forward
• 3′ sequencing primer: BGH Reverse

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The inactivating D343E and D345E were identified by Yang et al., 1994, J Biol Chem. 1994 Apr 22;269(16):11859-68 and combined by Repasky et al., 2004, doi: 10.4049/jimmunol.172.9.5478. Please visit https://doi.org/10.1101/639120 for bioRxiv preprint.

DNA (Catalog # 126455-D50)

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

• Amount: 50 μg
• Concentration: 1 μg/μL
• Pricing: $495 USD
• Storage: 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

DNA (Catalog # 126455-D100)

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

• Amount: 100 μg
• Concentration: 1 μg/μL
• Pricing: $585 USD
• Storage: 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.