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TrmD_HaeIn/pET28b(+) N,C-tag
(Plasmid #12665)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 12665 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pET-28b(+)
  • Backbone manufacturer
    Merck
  • Backbone size w/o insert (bp) 5369
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    tRNA (guanine-N(1)-)-methyltransferase
  • Alt name
    TrmD
  • Species
    Haemophilus influenzae
  • Insert Size (bp)
    741
  • GenBank ID
    L42023
  • Entrez Gene
    trmD
  • Tags / Fusion Proteins
    • His (N terminal on insert)
    • His (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    TrmD_HaeIn/pET28b(+) N,C-tag was a gift from SeWon Suh (Addgene plasmid # 12665 ; http://n2t.net/addgene:12665 ; RRID:Addgene_12665)
  • For your References section:

    Crystallization and preliminary X-ray crystallographic analysis of tRNA(m1G37)methyltransferase from Haemophilus influenzae. Kim HW, Ahn HJ, Yoon HJ, Kim HW, Baek SH, Suh SW. Acta Crystallogr D Biol Crystallogr. 2003 Jan . 59(Pt 1):183-4. 10.1107/S0907444902019716 PubMed 12499565