Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more


TrmD_HaeIn/pET28b(+) N,C-tag
(Plasmid #12665)


Item Catalog # Description Quantity Price (USD)
Plasmid 12665 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
  • Backbone size w/o insert (bp) 5369
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    Low Copy


  • Gene/Insert name
    tRNA (guanine-N(1)-)-methyltransferase
  • Alt name
  • Species
    Haemophilus influenzae
  • Insert Size (bp)
  • GenBank ID
  • Entrez Gene
  • Tags / Fusion Proteins
    • His (N terminal on insert)
    • His (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    TrmD_HaeIn/pET28b(+) N,C-tag was a gift from SeWon Suh (Addgene plasmid # 12665 ; ; RRID:Addgene_12665)
  • For your References section:

    Crystallization and preliminary X-ray crystallographic analysis of tRNA(m1G37)methyltransferase from Haemophilus influenzae. Kim HW, Ahn HJ, Yoon HJ, Kim HW, Baek SH, Suh SW. Acta Crystallogr D Biol Crystallogr. 2003 Jan . 59(Pt 1):183-4. 10.1107/S0907444902019716 PubMed 12499565