EF1a-TALEN_HD (SPC right)
(Plasmid #126695)

• Purpose: right TALENS for introducing a double strand break into the genomic human SFTPC locus at the ATG start site
• Depositing Lab: Darrell Kotton
• Publication: Jacob et al Cell Stem Cell. 2017 Oct 5;21(4):472-488.e10. doi: 10.1016/j.stem.2017.08.014. Epub 2017 Sep 28.

Backbone

• Vector backbone: EF1a-TALEN
• Backbone manufacturer: Derived in Kotton lab
• Backbone size w/o insert (bp): 6413
• Vector type: Mammalian Expression, TALEN

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: human SPC right
• Insert Size (bp): 1897
• Mutation: R451P in SPC
• Promoter: EF1
• Tags / Fusion Proteins:
  • NLS, truncated N term (N terminal on insert)
  • TAL, FokI (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Unknown (unknown if destroyed)
• 3′ cloning site: Unknown (unknown if destroyed)

Resource Information

• Supplemental Documents:
  • p1310 EF1-TALEN_HD (SPC Right).gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note that the Addgene verified sequence finds an R451P mutation in SPC and a deletion of the 0.5 repeat HD region when compared to the depositor's sequence. These differences do not impact plasmid function.