Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

Differentiation of Human Pluripotent Stem Cells into Functional Lung Alveolar Epithelial Cells.
Jacob A, Morley M, Hawkins F, McCauley KB, Jean JC, Heins H, Na CL, Weaver TE, Vedaie M, Hurley K, Hinds A, Russo SJ, Kook S, Zacharias W, Ochs M, Traber K, Quinton LJ, Crane A, Davis BR, White FV, Wambach J, Whitsett JA, Cole FS, Morrisey EE, Guttentag SH, Beers MF, Kotton DN
Cell Stem Cell. 2017 Oct 5;21(4):472-488.e10. doi: 10.1016/j.stem.2017.08.014. Epub 2017 Sep 28.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
126694EF1a-TALEN_NN (SPC left)left TALENS for introducing a double strand break into the genomic human SFTPC locus at the ATG start site
126696p1303 DV-SFTPC-tdTomatodonor vector for targeting a tdTomato reporter to the human SFTPC locus at the endogenous ATG start site
126700pHAGE2-EF1aL-Cre-IRES-NeoR-Wlentiviral or plasmid based co-expression of Cre recombinase and a neomycin resistance cassette