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pHAGE-UBC-hAAT-IRES-ZsGreen-W
(Plasmid #126702)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 126702 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pHAGE
  • Backbone manufacturer
    Derived in Kotton lab
  • Backbone size w/o insert (bp) 6256
  • Vector type
    Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Alpha-1 antitrypsin (hAAT aka SERPINA1) and ZsGreen; "AAT-IRES-ZSGreen"
  • Alt name
    SERPINA1
  • Alt name
    a1AT
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2528
  • Entrez Gene
    SERPINA1 (a.k.a. A1A, A1AT, AAT, PI, PI1, PRO2275, alpha1AT, nNIF)
  • Promoter UBC

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NotI (not destroyed)
  • 3′ cloning site ClaI (not destroyed)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHAGE-UBC-hAAT-IRES-ZsGreen-W was a gift from Darrell Kotton (Addgene plasmid # 126702 ; http://n2t.net/addgene:126702 ; RRID:Addgene_126702)
  • For your References section:

    Sustained expression of alpha1-antitrypsin after transplantation of manipulated hematopoietic stem cells. Wilson AA, Kwok LW, Hovav AH, Ohle SJ, Little FF, Fine A, Kotton DN. Am J Respir Cell Mol Biol. 2008 Aug . 39(2):133-41. 10.1165/rcmb.2007-0133OC PubMed 18323534