Pk_CyRPA-Cd4-bio-His
(Plasmid
#126841)
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PurposeExpression of recombinant P. knowlesi protein in mammalian cells
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Depositing Lab
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Depositing OrganizationWellcome Sanger Institute
Located in United Kingdom of Great Britain and Northern Ireland -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 126841 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 126841-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 126841-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepTT3
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Backbone manufacturerYves Durocher, NRC
- Backbone size w/o insert (bp) 6688
- Total vector size (bp) 7719
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCyRPA
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Alt namePKNH_0515800
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Alt namePKH_052740
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SpeciesPlasmodium knowlesi
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Insert Size (bp)1031
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GenBank IDCAQ38826.1
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Entrez GenePKH_052740 (a.k.a. PKH_052740)
- Promoter CMV
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Tag
/ Fusion Protein
- ratCd4(d3+4)-bio-6His (C terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NotI (not destroyed)
- 3′ cloning site AscI (not destroyed)
- 5′ sequencing primer CMV-F
- (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byInsert was chemically synthesised.
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 126841-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 126841-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Pk_CyRPA-Cd4-bio-His was a gift from Gavin Wright (Addgene plasmid # 126841 ; http://n2t.net/addgene:126841 ; RRID:Addgene_126841) -
For your References section:
A panel of recombinant proteins from human-infective Plasmodium species for serological surveillance. Muller-Sienerth N, Shilts J, Kadir KA, Yman V, Homann MV, Asghar M, Ngasala B, Singh B, Farnert A, Wright GJ. Malar J. 2020 Jan 17;19(1):31. doi: 10.1186/s12936-020-3111-5. 10.1186/s12936-020-3111-5 PubMed 31952523
Map uploaded by the depositor.
