pZF-AAA
(Plasmid #127465)

• Purpose: coding sequence for wild type zinc finger ZF-AAA (ZiF268)
• Depositing Lab: Sebastian Maerkl
• Publication: Swank et al Proc Natl Acad Sci U S A. 2019 Mar 26;116(13):5892-5901. doi: 10.1073/pnas.1816591116. Epub 2019 Mar 8.

Backbone

• Vector backbone: pET-21a
• Backbone size w/o insert (bp): 5344
• Total vector size (bp): 5607
• Vector type: cell-free transcription–translation

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: ZF-AAA
• Alt name: ZiF268
• Species: H. sapiens (human)
• Insert Size (bp): 267
• Entrez Gene: EGR1 (a.k.a. AT225, G0S30, KROX-24, NGFI-A, TIS8, ZIF-268, ZNF225)
• Promoter: T7

Cloning Information

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: ATGCGTCCGGCGTAG
• 3′ sequencing primer: AACCCCTCAAGACCCG

Resource Information

• Supplemental Documents:
  • ZF_AAA.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Integrating Gene Synthesis and Microfluidic Protein Analysis for Rapid Protein Engineering. NAR. 2016 April; 44(7): e68. doi:10.1093/nar/gkv1497.