pSH87
(Plasmid
#127705)
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PurposePflp-13::gfp1-10
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 127705 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonePflp-13
- Backbone size w/o insert (bp) 5675
- Total vector size (bp) 6317
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Vector typeWorm Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameGFP1-10
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SpeciesSynthetic
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Insert Size (bp)642
- Promoter flp-13
Cloning Information
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer TCCAAGCTTGCATGCAAATTGTGCCTCCTGATG
- 3′ sequencing primer CTCTAGAGGCGCGCcGTTCGAATCGGAACGGG (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSH87 was a gift from David M. Miller (Addgene plasmid # 127705 ; http://n2t.net/addgene:127705 ; RRID:Addgene_127705) -
For your References section:
NATF (Native And Tissue-Specific Fluorescence): A Strategy for Bright, Tissue-Specific GFP Labeling of Native Proteins in Caenorhabditis elegans. He S, Cuentas-Condori A, Miller DM. Genetics. 2019 Apr 5. pii: genetics.119.302063. doi: 10.1534/genetics.119.302063. 10.1534/genetics.119.302063 PubMed 30952669
