pLPT234
(Plasmid #127855)

• Purpose: Repressilator 2.0, without degradation tags, integrated triple reporter
• Depositing Lab: Johan Paulsson
• Publication: Riglar et al Nat Commun. 2019 Oct 11;10(1):4665. doi: 10.1038/s41467-019-12638-z.

Backbone

• Vector backbone: pSC101
• Backbone size w/o insert (bp): 3000
• Total vector size (bp): 9745
• Vector type: Bacterial Expression, Synthetic Biology

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert 1

• Gene/Insert name: bla
• Alt name: AmpR
• Species: E. coli
• Insert Size (bp): 861
• Promoter: pAmpR

Cloning Information for Gene/Insert 1

• Cloning method: Unknown
• 5′ sequencing primer: n/a

Gene/Insert 2

• Gene/Insert name: mSCFP3
• Insert Size (bp): 723
• Promoter: pL LacO1

Cloning Information for Gene/Insert 2

• Cloning method: Gibson Cloning
• 5′ sequencing primer: n/a

Gene/Insert 3

• Gene/Insert name: mKate2
• Insert Size (bp): 723
• Mutation: First 11aa derived from mCherry as fusion protein
• Promoter: pR

Cloning Information for Gene/Insert 3

• Cloning method: Gibson Cloning
• 5′ sequencing primer: n/a

Gene/Insert 4

• Gene/Insert name: mVenus
• Insert Size (bp): 717
• Promoter: pL tetO1

Cloning Information for Gene/Insert 4

• Cloning method: Gibson Cloning
• 5′ sequencing primer: n/a

Gene/Insert 5

• Gene/Insert name: TetR
• Insert Size (bp): 624
• Promoter: pL LacO1

Cloning Information for Gene/Insert 5

• Cloning method: Unknown
• 5′ sequencing primer: n/a

Gene/Insert 6

• Gene/Insert name: LacI
• Insert Size (bp): 1083
• Promoter: pR

Cloning Information for Gene/Insert 6

• Cloning method: Unknown
• 5′ sequencing primer: n/a

Gene/Insert 7

• Gene/Insert name: cI
• Alt name: lambda repressor
• Species: lambda phage
• Promoter: pL tetO1

Cloning Information for Gene/Insert 7

• Cloning method: Unknown
• 5′ sequencing primer: n/a

Resource Information

• Supplemental Documents:
  • pLPT234.gb
• A portion of this plasmid was derived from a plasmid made by: Original repressilator plasmid by Michael Elowitz (Elowitz and Leibler, 2000, Nature). Cloning for this plasmid by Laurent Potvin-Trottier and Johan Paulsson, Harvard Medical School.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Evrogen Limited Use Label License for FPs
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This plasmid is a derivative of repressilator plasmids presented in: Potvin-Trottier et al, 2016, Nature.