|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||26489||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 3552
Vector typeBacterial Expression
Growth in Bacteria
Growth instructionsplasmid is in MG1655Z1 which is spectR
Gene/Insert nameLacI, tetR
Insert Size (bp)6664
- Cloning method Restriction Enzyme
- 5′ cloning site XhoI (not destroyed)
- 3′ cloning site BamH1 (not destroyed)
- 5′ sequencing primer n/a (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:repressilator (pZS1-lTlrLLtCL) was a gift from Michael Elowitz (Addgene plasmid # 26489 ; http://n2t.net/addgene:26489 ; RRID:Addgene_26489)
For your References section:A synthetic oscillatory network of transcriptional regulators. Elowitz MB, Leibler S. Nature. 2000 Jan 20. 403(6767):335-8. 10.1038/35002125 PubMed 10659856