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(Plasmid #127867)


Item Catalog # Description Quantity Price (USD)
Plasmid 127867 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone size w/o insert (bp) 5341
  • Total vector size (bp) 5982
  • Vector type

Growth in Bacteria

  • Bacterial Resistance(s)
  • Growth Temperature
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy


  • Gene/Insert name
  • Alt name
  • Species
    H. sapiens (human), M. musculus (mouse)
  • Insert Size (bp)
  • Mutation
    constructed by blunt end fusion of the 5’ 51 nucleotides (starting from the ATG) of the human NOS1AP (short isoform) ORF, to the 3’ 590 nucleotides of the mouse NOS1AP
  • GenBank ID
    NM_001109985 NM_001164757
  • Entrez Gene
    Nos1ap (a.k.a. 6330408P19Rik, Capon)
  • Promoter hSyn
  • Tags / Fusion Proteins
    • mCherry (N terminal on backbone)
    • 3xFLAG (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer CCCCGTAATGCAGAAGAAGA
  • 3′ sequencing primer CATAGCGTAAAAGGAGCAACA
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    The backbone was obtained from pAAV-hSyn-hChR2(H134R)-mCherry, a gift from Karl Deisseroth (Addgene plasmid # 26976). mCherry was amplified by PCR from pAAV-hSyn-hChR2(H134R)-mCherry, a gift from Karl Deisseroth (Addgene plasmid # 26976).
  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV-hSyn-mCherry.3xFLAG.NOS1AP-S-WPRE was a gift from Florian Freudenberg (Addgene plasmid # 127867 ; ; RRID:Addgene_127867)