|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||1279||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector typeYeast Expression
Growth in Bacteria
Copy numberHigh Copy
Alt nameglucocorticoid receptor
SpeciesR. norvegicus (rat)
/ Fusion Protein
- FLAG (N terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site Nco I (not destroyed)
- 3′ cloning site Xho I (not destroyed)
- 5′ sequencing primer N/A (Common Sequencing Primers)
A portion of this plasmid was derived from a plasmid made bysee comments
Terms and Licenses
- Not Available to Industry
Derived from plasmids from the following reference: Picard D, Khurseed B, Garabedian MJ, Fortin MG, Lindquist S, Yamamoto
KR. Reduced levels of hsp90 compromise steroid receptor action in
vivo. Nature (1990), 348 (6297): 166-8.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCHFLAGGR was a gift from Susan Lindquist (Addgene plasmid # 1279 ; http://n2t.net/addgene:1279 ; RRID:Addgene_1279)
For your References section:Role of the protein chaperone YDJ1 in establishing Hsp90-mediated signal transduction pathways. Kimura Y, Yahara I, Lindquist S. Science 1995 Jun 2;268(5215):1362-5. 10.1126/science.7761857 PubMed 7761857