RFP Dynamin2 Wild Type
(Plasmid
#128152)
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PurposeExpression of Dynamin 2
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Depositing Lab
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Depositing OrganizationNational Institute of Child Health and Human Development (NICHD)
Located in the United States of America -
Citations
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 128152 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 128152-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 128152-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepEGFP
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameDynamin 2
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SpeciesR. norvegicus (rat)
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Entrez GeneDnm2 (a.k.a. DYIIAAB)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (unknown if destroyed)
- 3′ cloning site HindIII (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please note: Plasmid contains D406G, V664A, H692R, and Q758R mutations in Dynamin2 compared to the NCBI reference sequence XP_006242670.1. These mutations are not known to affect plasmid function.
DNA (Catalog # 128152-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 128152-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
RFP Dynamin2 Wild Type was a gift from Jennifer Lippincott-Schwartz (Addgene plasmid # 128152 ; http://n2t.net/addgene:128152 ; RRID:Addgene_128152) -
For your References section:
Dynamin 2 orchestrates the global actomyosin cytoskeleton for epithelial maintenance and apical constriction. Chua J, Rikhy R, Lippincott-Schwartz J. Proc Natl Acad Sci U S A. 2009 Dec 8;106(49):20770-5. doi: 10.1073/pnas.0909812106. Epub 2009 Nov 30. 10.1073/pnas.0909812106 PubMed 19948954
