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pGHUC w-ERBIN (2-hybrid prey plasmid)
(Plasmid #128163)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 128163 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    THE pGHUC
  • Backbone size w/o insert (bp) 7715
  • Total vector size (bp) 8042
  • Modifications to backbone
    Original backbone as published in: Oakes et al. Multi-reporter selection for the design of active and more specific zinc-finger nucleases for genome editing. Nature Communications. 2016. doi: 10.1038/ncomms10194. Modifications: Nonessential F1 ori replaced with UV5 promoter driving omega-ERBIN fusion. Sequence between EcoRI and AgeI replaced. Two copies of the preferred zif268 binding site were inserted consecutively, but in opposite orientations, 21bp upstream of the -35 boxes of relevant reporter promoters.
  • Vector type
    Bacterial Expression, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    ERBIN PDZ domain
  • Species
    Synthetic
  • Insert Size (bp)
    327
  • Entrez Gene
    ERBIN (a.k.a. ERBB2IP, HEL-S-78, LAP2)
  • Promoter UV5
  • Tags / Fusion Proteins
    • FLAG (N terminal on backbone)
    • Omega subunit of RNA polymerase (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XbaI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer CAAGAGCAGGAAGCCGCTG
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGHUC w-ERBIN (2-hybrid prey plasmid) was a gift from Marcus Noyes (Addgene plasmid # 128163 ; http://n2t.net/addgene:128163 ; RRID:Addgene_128163)
  • For your References section:

    A Multireporter Bacterial 2-Hybrid Assay for the High-Throughput and Dynamic Assay of PDZ Domain-Peptide Interactions. Ichikawa DM, Corbi-Verge C, Shen MJ, Snider J, Wong V, Stagljar I, Kim PM, Noyes MB. ACS Synth Biol. 2019 May 17;8(5):918-928. doi: 10.1021/acssynbio.8b00499. Epub 2019 Apr 18. 10.1021/acssynbio.8b00499 PubMed 30969105