pET28a-C9ORF80
(Plasmid #128418)

• Purpose: Express C9ORF80 in E. coli with a His tag (C-terminal)
• Depositing Lab: Yuliang Wu
• Publication: Vidhyasagar et al Biochem J. 2018 Jan 2;475(1):45-60. doi: 10.1042/BCJ20170351.

Backbone

• Vector backbone: pET28a
• Backbone manufacturer: Novagen
• Backbone size w/o insert (bp): 5369
• Total vector size (bp): 5545
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: C9ORF80
• Alt name: HSSBIP1
• Alt name: SOSS-C
• Alt name: INIP (INTS3 And NABP Interacting Protein)
• Species: H. sapiens (human)
• GenBank ID: NM_021218.3
• Entrez Gene: INIP (a.k.a. C9orf80, HSPC043, MISE, SOSSC, SSBIP1, hSSBIP1)
• Promoter: T7
• Tag / Fusion Protein:
  • His (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Nco I (not destroyed)
• 3′ cloning site: Xho I (not destroyed)
• 5′ sequencing primer: T7 promoter
• 3′ sequencing primer: T7 terminator

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry