Biochemical characterization of INTS3 and C9ORF80, two subunits of hNABP1/2 heterotrimeric complex in nucleic acid binding.
Vidhyasagar V, He Y, Guo M, Talwar T, Singh RS, Yadav M, Katselis G, Vizeacoumar FJ, Lukong KE, Wu Y
Biochem J. 2018 Jan 2;475(1):45-60. doi: 10.1042/BCJ20170351. PubMed Article
Vidhyasagar V, He Y, Guo M, Talwar T, Singh RS, Yadav M, Katselis G, Vizeacoumar FJ, Lukong KE, Wu Y
Biochem J. 2018 Jan 2;475(1):45-60. doi: 10.1042/BCJ20170351. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 128415 | pGEX-6P-1-INTS3-FL | Express INTS3 full-length in E. coli with a GST tag (N-terminal) |
| 128416 | pGEX-6P-1-INTS3-N | Express INTS3 N-termini (1-513 aa) in E. coli with a GST tag (N-terminal) |
| 128417 | pGEX-6P-1-INTS3-C | Express INTS3 C-termini (514-1042 aa) in E. coli with a GST tag (N-terminal) |
| 128418 | pET28a-C9ORF80 | Express C9ORF80 in E. coli with a His tag (C-terminal) |
| 128642 | pET28a-INTS3-C | Express INTS3 C-termini (514-1042 aa) in E. coli with N-terminal and C-terminal His tag |