pPHAGE-CR-PINK1-C-TAP
(Plasmid #128510)

• Purpose: Lentiviral constitutive expression of CRISPR-resistant PINK1 with c-terminal FLAG and HA tag.
• Depositing Lab: Mohan Babu
• Publication: Moutaoufik et al iScience. 2019 Sep 4;19:1114-1132. doi: 10.1016/j.isci.2019.08.057.

Backbone

• Vector backbone: pPHAGE C-TAP
• Backbone size w/o insert (bp): 10056
• Total vector size (bp): 9593
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: PTEN induced kinase 1
• Species: H. sapiens (human)
• Mutation: Synonymous silent mutation in Q5 to render sgRNA PAM site silent
• Entrez Gene: PINK1 (a.k.a. BRPK, PARK6)
• Promoter: CMV
• Tag / Fusion Protein:
  • FLAG and HA tags (C terminal on insert)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: CMV-F CGCAAATGGGCGGTAGGCGTG, ATCCACGCTGTTTTGACCTC
• 3′ sequencing primer: AAGAAGCGGAGACGGTTAGG

Resource Information

• Supplemental Documents:
  • pPhage_CMV_CR-PINK1_C_FLAG_HA_IRES_Puro.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry