pMJA180
(Plasmid #128538)

• Purpose: Expresses E coli Bir A protein
• Depositing Lab: Marcos Alcocer
• Publication: de la Cruz et al J Biotechnol. 2016 Jun 10;227:112-9. doi: 10.1016/j.jbiotec.2016.04.024. Epub 2016 Apr 13.

Backbone

• Vector backbone: pPIC6alpha
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 3607
• Vector type: Yeast Expression
• Selectable markers: Blasticidin

Growth in Bacteria

• Bacterial Resistance(s): Blasticidin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: E coli BirA
• Alt name: DF192_21550
• Species: Escherichia coli
• Insert Size (bp): 967
• Promoter: AOX1

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: SacII (not destroyed)
• 5′ sequencing primer: CGACTGGTTCCAATTGACAAGCTTT
• 3′ sequencing primer: TGCATCTCTCAGGCAAATGGCA

Resource Information

• Supplemental Documents:
  • pMJA180=pPIC6 alpha A+Ecoli BirA.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

secreted E coli BirA a biotinylation synthetase protein in Pichia