pQCXIpuro-H3.3K4M-FLAG/HA
(Plasmid #128742)

• Purpose: Expression of histone H3.3K4M-FLAG/HA
• Depositing Lab: Kai Ge
• Publication: Jang et al Nucleic Acids Res. 2019 Jan 25;47(2):607-620. doi: 10.1093/nar/gky982.

Backbone

• Vector backbone: pQCXIPpuro
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 553
• Total vector size (bp): 7200
• Vector type: Retroviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: H3.3K4M
• Species: H. sapiens (human)
• Mutation: K4M
• GenBank ID: NM_002107.6
• Entrez Gene: H3-3A (a.k.a. BRYLIB1, H3-3B, H3.3A, H3F3, H3F3A)
• Promoter: CMV
• Tag / Fusion Protein:
  • FLAG and HA (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XhoI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: CCGGGAACGGTGCATT
• 3′ sequencing primer: CGGATTGCTCGTCAAG

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry