pACYCDuet-1_Ec_rpoZ
(Plasmid
#128837)
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PurposeE. coli rpoz gene cloned between NcoI and HindIII sites of the first MCS of this Duet plasmid. Omega protein.
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 128837 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepACYCDuet-1
- Backbone size w/o insert (bp) 3939
- Total vector size (bp) 4215
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol, 25 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namerpoZ
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SpeciesE. coli
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Insert Size (bp)276
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Entrez GenerpoZ (a.k.a. b3649, ECK3639, spoS)
- Promoter T7
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NcoI (not destroyed)
- 3′ cloning site HindIII (not destroyed)
- 5′ sequencing primer T7 Promotor
- 3′ sequencing primer T7 Terminator (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pACYCDuet-1_Ec_rpoZ was a gift from Seth Darst (Addgene plasmid # 128837 ; http://n2t.net/addgene:128837 ; RRID:Addgene_128837) -
For your References section:
X-ray Crystal Structure of Escherichia coli RNA Polymerase sigma70 Holoenzyme. Murakami KS. J Biol Chem. 2013 Mar 29;288(13):9126-34. doi: 10.1074/jbc.M112.430900. Epub 2013 Feb 6. 10.1074/jbc.M112.430900 PubMed 23389035
Map uploaded by the depositor.
