pGEMABC
(Plasmid #45398)

• Depositing Lab: Katsu Murakami
• Publication: Murakami et al J Biol Chem. 2013 Mar 29;288(13):9126-34. doi: 10.1074/jbc.M112.430900. Epub 2013 Feb 6.

Backbone

• Vector backbone: pGEMEX1
• Backbone manufacturer: Promega
• Backbone size w/o insert (bp): 3993
• Total vector size (bp): 12737
• Modifications to backbone: Digesting pGEMEX1 (Promega) with Xbal and recircularizing the larger fragment. By this treatment, the T7 gene 10 coding for major capsid protein was deleted.
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: rpoA
• Alt name: alpha subunit RNA polymerase
• Species: Escherichia coli
• Insert Size (bp): 987
• Entrez Gene: rpoA (a.k.a. Y75_p3881)
• Promoter: T7

Cloning Information for Gene/Insert 1

• Cloning method: Restriction Enzyme
• 5′ cloning site: XbaI (not destroyed)
• 3′ cloning site: XbaI (not destroyed)
• 5′ sequencing primer: T7 primer
• 3′ sequencing primer: None

Gene/Insert 2

• Gene/Insert name: rpoB
• Alt name: beta subunit RNA polymerase
• Species: Escherichia coli
• Insert Size (bp): 4026
• Entrez Gene: rpoB (a.k.a. Y75_p3210)
• Promoter: None

Cloning Information for Gene/Insert 2

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: None (unknown if destroyed)
• 5′ sequencing primer: None
• 3′ sequencing primer: None

Gene/Insert 3

• Gene/Insert name: rpoC
• Alt name: betaprime subunit RNA polymerase
• Species: Escherichia coli
• Insert Size (bp): 4221
• Entrez Gene: rpoC (a.k.a. Y75_p3209)
• Promoter: None

Cloning Information for Gene/Insert 3

• Cloning method: Restriction Enzyme
• 5′ cloning site: None (unknown if destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: None
• 3′ sequencing primer: T7 terminator

Resource Information

• Articles Citing this Plasmid:
  • 6 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry