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pMV10
(Plasmid #128892)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 128892 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pUC57BsaI
  • Backbone size w/o insert (bp) 2724
  • Total vector size (bp) 3846
  • Modifications to backbone
    Insertion of AGCA-URA3-CCAT by MEGAWHOP
  • Vector type
    Yeast Expression, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    URA3
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    1122
  • Entrez Gene
    URA3 (a.k.a. YEL021W)

Resource Information

Depositor Comments

Please visit https://www.biorxiv.org/content/10.1101/630277v1 for BioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMV10 was a gift from Gilles Charvin (Addgene plasmid # 128892 ; http://n2t.net/addgene:128892 ; RRID:Addgene_128892)
  • For your References section:

    COSPLAY: An expandable toolbox for combinatorial and swift generation of expression plasmids in yeast. Goulev Y, Matifas A, Heyer V, Reina-San-Martin B, Charvin G. PLoS One. 2019 Aug 28;14(8):e0220694. doi: 10.1371/journal.pone.0220694. eCollection 2019. 10.1371/journal.pone.0220694 PubMed 31461461