COSPLAY: An expandable toolbox for combinatorial and swift generation of expression plasmids in yeast.
Goulev Y, Matifas A, Heyer V, Reina-San-Martin B, Charvin G
PLoS One. 2019 Aug 28;14(8):e0220694. doi: 10.1371/journal.pone.0220694. eCollection 2019. PubMed Article
Goulev Y, Matifas A, Heyer V, Reina-San-Martin B, Charvin G
PLoS One. 2019 Aug 28;14(8):e0220694. doi: 10.1371/journal.pone.0220694. eCollection 2019. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 128859 | pUC57BsaI | Module cloning vector |
| 128860 | pDV | Destination vector for modules assembly |
| 128861 | pMV14 | Position 1 module -2 microns |
| 128863 | pMV8 | Position 1 module- Linker |
| 128864 | pMV13 | Position 1 module- CEN (Centromeric) |
| 128865 | pMV46 | Position 1 module- VI260998 (yeast integrative region VI260998) |
| 128866 | pMV47 | Position 1 module- IX301997 (yeast integrative region IX301997) |
| 128867 | pMV1 | Position 2 module- Gal1 promoter |
| 128876 | pMV2 | Position 2 module- Met25 promoter |
| 128877 | pMV17 | Position 2 module- Act1 promoter |
| 128878 | pMV19 | Position 2 module- Adh1 promoter |
| 128879 | pMV20 | Position 2 module- Cyc1 promoter |
| 128880 | pMV21 | Position 2 module- Tef1 promoter |
| 128881 | pMV48 | Position 2 module- Tdh3 promoter |
| 128882 | pMV3 | Position 3 module- sfGFP |
| 128883 | pMV4 | Position 3 module- mCherry |
| 128884 | pMV22 | Position 3 module- Venus |
| 128885 | pMV45 | Position 3 module- 3SV40NLS (3x NLS from SV40) |
| 128886 | pMV5 | Position 4 module- CLN2-PEST (Degron) |
| 128887 | pMV23 | Position 4 module- STOP (stop codon) |
| 128888 | pMV42 | Position 4 module- mCherry-STOP |
| 128889 | pMV44 | Position 4 module- sfGFP-STOP |
| 128890 | pMV6 | Position 5 module- Adh1 terminator |
| 128891 | pMV7 | Position 6 module- TRP1 |
| 128892 | pMV10 | Position 6 module- URA3 |
| 128893 | pMV12 | Position 6 module- NatMX |
| 128894 | pMV16 | Position 6 module- LEU2 |
| 128895 | pMV18 | Position 6 module- KanMX |