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PurposeInducible overexpression of NFIA and Sox9 in human cells.
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Depositing Lab
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Depositing OrganizationUniversity of Wisconsin, Madison
Located in the United States of America -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 129455 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 129455-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 129455-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backboneAAVS1-TRE3G
- Backbone size w/o insert (bp) 11159
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Vector typeMammalian Expression
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSox 9
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Alt nameCMD1; SRA1; CMPD1; SRXX2; SRXY10
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SpeciesH. sapiens (human)
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Insert Size (bp)1530
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GenBank ID6662
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Entrez GeneSOX9 (a.k.a. CMD1, CMPD1, ENH13, SRA1, SRXX2, SRXY10, TESCO)
- Promoter TRE3G
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site SalI (unknown if destroyed)
- 3′ cloning site MluI (unknown if destroyed)
- 5′ sequencing primer ACCCGCACTTGCACAACG
- 3′ sequencing primer GGTGGTCCTTCTTGTGCTG
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 129455-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 129455-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
AAVS1-TRE3G-NFIA+SOX9 was a gift from Su-Chun Zhang (Addgene plasmid # 129455 ; http://n2t.net/addgene:129455 ; RRID:Addgene_129455) -
For your References section:
Fast Generation of Functional Subtype Astrocytes from Human Pluripotent Stem Cells. Li X, Tao Y, Bradley R, Du Z, Tao Y, Kong L, Dong Y, Jones J, Yan Y, Harder CRK, Friedman LM, Bilal M, Hoffmann B, Zhang SC. Stem Cell Reports. 2018 Oct 9;11(4):998-1008. doi: 10.1016/j.stemcr.2018.08.019. Epub 2018 Sep 27. 10.1016/j.stemcr.2018.08.019 PubMed 30269954
