AAVS1-TRE3G-NFIA+SOX9
(Plasmid #129455)

• Purpose: Inducible overexpression of NFIA and Sox9 in human cells.
• Depositing Lab: Su-Chun Zhang
• Publication: Li et al Stem Cell Reports. 2018 Oct 9;11(4):998-1008. doi: 10.1016/j.stemcr.2018.08.019. Epub 2018 Sep 27.

Backbone

• Vector backbone: AAVS1-TRE3G
• Backbone size w/o insert (bp): 11159
• Vector type: Mammalian Expression
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Sox 9
• Alt name: CMD1; SRA1; CMPD1; SRXX2; SRXY10
• Species: H. sapiens (human)
• Insert Size (bp): 1530
• GenBank ID: 6662
• Entrez Gene: SOX9 (a.k.a. CMD1, CMPD1, SRA1, SRXX2, SRXY10)
• Promoter: TRE3G

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SalI (unknown if destroyed)
• 3′ cloning site: MluI (unknown if destroyed)
• 5′ sequencing primer: ACCCGCACTTGCACAACG
• 3′ sequencing primer: GGTGGTCCTTCTTGTGCTG

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Tet Systems Limited Use Label License
• Industry Terms:
  • Not Available to Industry