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pIRES2-hPAC-FLAG
(Plasmid #129494)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 129494 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pIRES2-EGFP
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 5300
  • Total vector size (bp) 6377
  • Modifications to backbone
    Insert hPAC C-FLAG
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    human PAC tagged with FLAG tag at the C-terminal end
  • Alt name
    C-terminus FLAG-tagged hPAC
  • Alt name
    hPAC C-FLAG
  • Alt name
    TMEM206
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1077
  • GenBank ID
    NP_060722
  • Entrez Gene
    PACC1 (a.k.a. C1orf75, PAC, TMEM206)
  • Promoter CMV
  • Tag / Fusion Protein
    • FLAG (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (unknown if destroyed)
  • 3′ cloning site EcoRI (unknown if destroyed)
  • 5′ sequencing primer CMV - F
  • 3′ sequencing primer IRES - Rev
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pIRES2-hPAC-FLAG was a gift from Zhaozhu Qiu (Addgene plasmid # 129494 ; http://n2t.net/addgene:129494 ; RRID:Addgene_129494)
  • For your References section:

    PAC, an evolutionarily conserved membrane protein, is a proton-activated chloride channel. Yang J, Chen J, Del Carmen Vitery M, Osei-Owusu J, Chu J, Yu H, Sun S, Qiu Z. Science. 2019 Apr 26;364(6438):395-399. doi: 10.1126/science.aav9739. Epub 2019 Apr 25. 10.1126/science.aav9739 PubMed 31023925