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(Plasmid #129554)


Item Catalog # Description Quantity Price (USD)
Plasmid 129554 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Total vector size (bp) 10973
  • Modifications to backbone
    mutation G8694C to remove BbsI site from pSG5 rep
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Apramycin, 25 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
  • Alt name
    Francisella tularensis subsp. Novicida U112 Cpf1
  • Insert Size (bp)
  • Promoter rpsL(XC)-BbsI

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCRISPomyces-FnCpf1 was a gift from Fong Tian Wong & Mingzi M. Zhang (Addgene plasmid # 129554 ; ; RRID:Addgene_129554)
  • For your References section:

    Characterization of Cas proteins for CRISPR-Cas editing in streptomycetes. Yeo WL, Heng E, Tan LL, Lim YW, Lim YH, Hoon S, Zhao H, Zhang MM, Wong FT. Biotechnol Bioeng. 2019 May 15. doi: 10.1002/bit.27021. 10.1002/bit.27021 PubMed 31090220